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首页> 外文OA文献 >Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies
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Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies

机译：从储油库中分离出与地下嗜热厌氧菌系统相关的新型嗜热厌氧菌：将地下嗜热厌氧杆菌，永生嗜热厌氧杆菌，腾热嗜热厌氧杆菌和太平洋羧二歧杆菌重新分配给地下嗜热杆菌。十一月，sp。十一月，梳。十一月作为四个新的亚种

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摘要

Novel thermophilic, anaerobic, Gram-positive, rod-shaped bacteria, strains SL9 and OCA1, were isolated from oilfields in France and Australia, respectively. Both strains, together with KB-1 (=DSM 13777), MB4 (=DSM 15242) and JM (=DSM 12653), possessed genomic (DNA–DNA hybridization studies) and phylogenetic similarities with SEBR 7858 (=DSM 13054), which was isolated recently from an oilfield reservoir in south-west France. Marked phenotypic differences exist between the three oilfield isolates (, strain OCA1 and strain SL9): they include temperature range for growth and substrates used. Differences were also observed in the DNA G+C contents of all organisms. Similarly to , strains SL9 and OCA1, and also , and , produced acetate and -alanine as major end products of glucose metabolism [0·8–1·0 mol -alanine produced (mol glucose consumed)] and reduced thiosulfate, but not sulfate, to sulfide. Because of these significant metabolic and phylogenetic differences between the oilfield isolates (, strain OCA1 and strain SL9), , and and other species, it is proposed to reassign them as a novel genus and species, gen. nov., sp. nov., comb. nov., with the creation of four novel subspecies, subsp. subsp. nov., comb. nov., subsp. subsp. nov., comb. nov., subsp. subsp. nov., comb. nov. and subsp. subsp. nov., comb. nov.
机译：分别从法国和澳大利亚的油田中分离出了新型嗜热，厌氧，革兰氏阳性杆状细菌SL9和OCA1菌株。两种菌株，连同KB-1（= DSM 13777），MB4（= DSM 15242）和JM（= DSM 12653），均具有基因组（DNA-DNA杂交研究）和与SEBR 7858（= DSM 13054）的系统发育相似性。最近是从法国西南部的一个油田中分离出来的。在三种油田分离物（OCA1菌株和SL9菌株）之间存在明显的表型差异：它们包括生长温度范围和所用底物。还观察到所有生物的DNA G + C含量存在差异。类似于SL9和OCA1菌株，以及和产生的乙酸盐和-丙氨酸是葡萄糖代谢的主要最终产物[产生0·8–1·0 mol-丙氨酸（消耗的葡萄糖摩尔数）]，并还原了硫代硫酸盐，但未还原硫酸盐，以硫化。由于这些油田分离株（OCA1株和SL9株）与其他物种之间存在明显的代谢和系统发育差异，因此建议将它们重新分配为新的属和种。十一月，sp。十一月，梳。十一月，创造了四个新的亚种，亚种。亚种十一月，梳。十一月。亚种十一月，梳。十一月。亚种十一月，梳。十一月和子。亚种十一月，梳。十一月

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